SAM50 complex inserts proteins into mitochondrial outer membrane

As inferred from the yeast SAM50 complex, the human SAMM50 Complex (SAM50 complex, TOB55 complex) inserts mainly beta-barrel proteins into the outer membrane after they have passed from the cytosol, through the TOMM40:TOMM70 complex, and into the intermembrane space.<br>In yeast, experimentally verified substrates of the SAM50 complex include TOM40 (TOMM40 in human), MDM10, Porin1 (VDAC1 in human), and TOM22 (TOMM22 in human). In humans, TOMM40 (Humphries et al. 2005) and VDAC1 (Kozjak-Pavlovic et al. 2007, homologous to yeast Porin1) have been shown to be substrates. Many other mitochondrial proteins are anticipated to be substrates of the SAMM50 complex.

据酵母SAM50复合体推断，人类SAMM50复合体（SAM50复合体，TOB55复合体）主要在蛋白从细胞质通过TOMM40:TOMM70复合体进入内膜空间后，将其β- barrel蛋白插入到外膜。在酵母中，经实验验证的SAM50复合体的底物包括TOM40（人类中的TOMM40）、MDM10、Porin1（人类中的VDAC1）以及TOM22（人类中的TOMM22）。在人类中，TOMM40（Humphries等，2005年）和VDAC1（Kozjak-Pavlovic等，2007年，与酵母Porin1同源）已被证实为底物。预计许多其他线粒体蛋白也可能是SAMM50复合体的底物。