Age-related gene expression changes in seeded tauopathy

Wildtype mice at either 3 or 12 months old were subjected to intrahippocampal injection with sarkosyl insoluble brain extracts from either human control or Alzheimer's disease brain. Mice were incubated for 1, 3 or 5 months and then sacrificed. The hippocampus from one hemisphere was used to extract total RNA that was then subjected to polyA-selection and whole transcriptome sequencing. 3 and 12 month old animals were processed in seperate batches and comparisons between control and Alzheimer's disease injected animals from each timepoint were conducted whole mouse transcriptome analysis of different timepoints and ages of mice with and without tauopathy