Genome-wide maps of Qki-5 and PPARb in mouse oligodendrocytes

We found that there is a big overlap between the Qki5- and PPARb-binding events by ChIP-seq. Interestingly, the genomic distribution analyses of Qki-5- and PPARβ-binding events showed significant enrichment at the regions of the promoter/transcription start site (TSS) which were indicated by Pol II-binding events. The strong preference of the promoter/TSS co-occupancy suggested that Qki-5 might play an important role in PPARβ-mediated transcriptional regulation of its downstream genes. Examination of genome-wide maps Qki-5 and PPARb in oligodendrocytes which are differentiated from neural stem cells or oligodendrocytes freshly isolated from mouse brains by ChIP-seq. Neural stem cells were differentiated in Neurobasal medium containing B-27, 2 mM GlutaMAX-I, 100 ng/mL insulin-like growth factor 1 (IGF-1), and 50 ng/mL 3,3',5-Triiodo-L-thyronine (T3). Oligodendrocytes were freshly isolated from mouse brains using O4 magnetic beads.