16S rRNA sequencing of gut microbiota in antibiotic-induced diarrhea mice

This project focuses on analyzing the gut microbiota composition and diversity in mice with antibiotic-associated diarrhea (AAD) using 16S rRNA gene (V3-V4 hypervariable region) high-throughput sequencing. A total of 15 mice were divided into: 1) Control group; 2) Antibiotic-associated diarrhea (AAD) model group; 3) AAD model + Zinc preparation group; 4) AAD model + Bifico group; 5) AAD model + Combination therapy group. The sequencing data will be used to compare the differences in gut microbiota structure among the five groups, identify key differential taxa, and explore the potential correlation between gut microbiota alterations and AAD, as well as the regulatory effects of different interventions on gut microbiota.