The dual role of Irf1 in maintaining epithelial identity while enabling EMT [ChIP-seq]

To investigate the context-dependent function of Irf1 in maintaining epithelial identity while enabling TGFbeta-induced EMT in NMuMG/E9 cells, we performed chromatin immunoprecipitation with Irf1-specific antibodies in NMuMG cells treated for 2 days with TGFbeta or left untreated (0d TGFbeta). Intersection with RNA-sequencing after downregulation of Irf1 with or without treatment with TGFbeta for two days revealed genes that are directly regulated by Irf1 and that could contribute to the dual role of Irf1 in EMT. ChIP sequencing of NMuMG/E9 cells with or without TGFbeta treatment (0d, 2d TGFb) was performed with Irf1-specific antibodies.