Expression data from mice normal MΦs and multiple myeloma tumor-associated macrophages (TAMs)

TAMs play an important role in MM drug resistance and progression. To determine the mechanisms about how tumor microenvironment regulate the generation of TAMs from normal MΦs in vitro and in vivo. We generated TAMs in vitro by tumor cells-MΦs coculture from CD36 WT and KO mice bone marrow cells. Total RNAs of 2 x 106 MΦs and TAMs were extracted by RNeasy Mini Kit (Qiagen). 5-10 µg RNA samples were sent to Gene Expression and Genotyping Facility at Case Western Reserve University (Cleveland, OH) for RNA-seq followed by data analysis. We use microarray data to determine differential expression of genes in between normal MΦs and TAMs. Examination of gene-profiling data in normal control MΦs and TAMs.