Comparasion between MCF7-NIK overexpressing cells, MCF7-control cells and MCF7-NIK depleted cells. Homo sapiens

Analysis of differentially expressed genes in MCF7 cancer cells transfected with an expression vector containing the ORF of NIK, a shRNA of NIK and a control shRNA. Transient transfections were performed in triplicates . We use micorarrays to elucidate the machanisms by which NIK regulates stem cells biology Overall design: Three groups were analyzed in triplicate each. The First group was MCF7 group· transfected with an expression vector that overexpress NIK, other group was MCF7 cells transfected with a shRNA to inhibit NIK and the last group was transfected with a shRNA to inhibit luciferase expression.