Comparison of gene expression by co-cultured WC1+ gammadelta and CD4+ alphabeta T cells. Bos taurus

Vaccination of animals with leptospiral vaccine results in the generation of antigen-responsive CD4 alphabeta T cells and WC1 gammadelta T cells which proliferate and produce effector cytokines following secondary encounter with antigen. In these studies we compared the gene expression of these antigen-stimulated T cell subsets to each other by microarray. In addition, each activated T cell population was compared to a reference sample, consisting of unstimulated peripheral blood mononuclear cells. Keywords: cell type comparison Overall design: CD4 T cells from 3 vaccinated animals compared to WC1 T cells from same animals, following in vitro antigen-stimulation. Each array was repeated for each animal using dye-swapped samples. CD4 or WC1 T cells from each vaccinated animal were also compared to ex vivo PBMC (unstimulated control). Here sample from antigen-stimulated CD4 or WC1 T cell was always Cy3 labelled, while unstimulated control was always Cy5 labelled.