Bacillus cereus strain:8A Genome sequencing and assembly. Bacillus cereus strain:8A

The identification of a bacteriocin with large activity spectrum, including pathogens and spoilage microorganisms, addresses an important aspect of food safety. A bacteriocin produced by the bacterium Bacillus cereus 8 A was identified. The antimicrobial activity was produced starting at the exponential growth phase, although maximum activity was at stationary growth phase. A crude bacteriocin obtained from culture supernatant fluid was inhibitory to a broad range of indicator strains, including Listeria monocytogenes, Clostridium perfringens , and several species of Bacillus. Clinically relevant bacteria such as Streptococcus bovis and Micrococcus luteus were also inhibited. Bacteriocin was stable at 80°C. It was resistant to the proteolytic action of trypsin and papain, but sensitive to proteinase K and pronaseE. Bacteriocin activity was observed in the pH range of 6.0–9.0