ITS sequencing of Mediterranean tree species

With the objective of analysing the relationship between health status and fungal diversity, in the present work the mycobiota of declined and healthy specimens of keystone Mediterranean tree species is described and compared. To this extent, bark and wood from close-to-natural Spanish forests of Castanea sativa (chestnut), Quercus ilex (holm oak), Quercus suber (cork oak) and Quercus pyrenaica (pyrenean oak) were sampled and the Internal Transcribed Spacer (ITS) genomic region was sequenced.