Sophisticated plant modification method through temporary gene expression
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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The present invention includes the step of temporarily expressing a sequence-specific nuclease specific for a target fragment in a cell or tissue of a target plant， wherein the sequence-specific nuclease is specific for the target site， and the target site is nucleus. It provides a method for carrying out site-specific modification in plants through transient expression of a gene， which is cleaved by a clease， whereby site-specific modification of the target site is achieved through DNA repair of the plant. Provided is a method for conducting site-specific modification in a plant through gene transient expression， comprising the following steps: transiently expressing a sequence-specific nuclease specific to the target fragment in the cell or tissue of the plant of interest， wherein the sequence-specific nuclease is specific to the target site and the target site is cleaved by the nuclease， thereby the site-specific modification of the target site is achieved through DNA repairing of the plant. Provided is a method for conducting site-specific modification in a plant through gene transient expression， comprising the following steps: transiently expressing a sequence-specific nuclease specific to the target fragment in the cell or tissue of the plant of interest， wherein the sequence-specific nuclease is specific to the target site and the target site is cleaved by the nuclease， thereby the site-specific modification of the target site is achieved through DNA repairing of the plant.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
