the transcriptome of Escherichia coli C321.delta A and EcNR2

Incorporation of noncanonical amino acids (ncAAs) into proteins has been proven to be a powerful tool to manipulate protein structure and function, and to investigate many biological processes. Improving the yields of ncAA-containing proteins is of great significance in industrial-scale applications. Escherichia coli C321.delta A was generated by the replacement of all known amber codons and the deletion of RF1 in the genome, and has been proven to be an ideal host for ncAA-containing protein expression. To obtain more information about the physiological and biochemical characteristics and reveal the potential effects of UAG code reassignment on E. coli C321.delta A, we analyzed the transcriptome and proteome of this strain