Transcriptome-based analysis reveals functional differences in Dendrobium officinale from different growing regions and with different quality levels

Dendrobium officinale is a common and expensive traditional Chinese medicine used as a medicinal agent and food that has immunoregulatory and anti-inflammatory effects. Methods to authenticate D. officinale are lacking. Although chemical profiles of D. officinale were published, transcriptomic profiling is lacking. Here, we collected D. officinale from two regions (Yunnan and Miandian) and the analogue D. compactum. The Yunnan samples had three different quality levels. All samples were subjected to genome-wide biological response fingerprinting (BioReF) in RAW264.7 cells using RNA sequencing. The analysis uses the comparison between the LPS treatment group and the DMSO control group as the model group, and the experimental samples treated with extracts of Dendrobium officinale are comparison with the LPS group , genes expression changes were determined by RNA-Seq to determine Dendrobium officinale responsive genes.