Transcriptome analysis of GmPUB20A overexpressing and RNA-interferencing transgenic hairy roots reveals the underlying negative role in soybean resistance to cyst nematode

To assay the transcriptional changes of transgenic soybean hairy roots that overexpression and RNA interferencing GmPUB20A, we constructed 15 mRNA libraries to RNA-seq.That's empty vector, GmPUB20A overexpression, GmPUB20A RNA interferencing, GmPUB20A overexpression innoculated with SCN and GmPUB20A RNA interferencing innoculated with SCN. Each treatment contained three bilogical replicates.