single-cell transcriptomes of meningeal and CP T cells in young and aged mice

The effects of aging on brain-associated T cells remain incompletely understood. We sorted CD4 and CD8 T cells from meninges and choroid plexus of young and aged mice, and compared their single-cell transcriptomes. The results provide insights into the changes of brain-associated T cells with aging, and identify unique aging-associated T cells. Overall design: Meninges and choroid plexus samples were obtained from young and aged mice. CD4 and CD8 T cells were sorted by FACS. scRNA-seq were performed using the 10xgenomics 5' gene expression kit. Libraries were sequenced by a NextSeq.