TMT-based proteomics of BMDMs following virus infection

To identify potential regulators involved in virus-triggered innate immune response, we used tandem mass tagging (TMT)-based shotgun proteomic analysis of murine bone marrow-derived macrophages (BMDMs) following sendai virus (SeV) infection. This approach facilitated the identification and relative quantification of up- or down-regulated proteins, as evaluated by replicates. Kyoto encyclopedia of genes and genomes (KEGG) analysis indicated that proteins involved in innate immune and inflammatory responses significantly increased following SeV infection. Surprisingly, we also observed a series of proteins involved in the regulation of cell cycle and DNA damage repair process were greatly impaired after SeV infection.