Secondary structure analyses of KCNE protein sequence to predict transmembrane helices.

For the first 4 methods, amino acid positions are given, For the Grand AVerage of hydropathicity (GRAVY) the calculated coefficients are given: a more positive value represents greater hydrophobicity[62]. 1PredictProtein at http://cubic.bioc.columbia.edu/predictprotein. 2MENSAT at http://saier-144-37.ucsd.edu/memsat.html. 3WaveTM at http://athina.biol.uoa.gr/bioinformatics/waveTM/. 4Statistical analysis of protein sequences at http://www.ebi.ac.uk/saps/. 5GRAVY coefficients of the transmembrane domains of KCNE1 and KCNE2 were calculated using ProtParam at http://ca.expasy.org/tools/protparam.html.