The impact of iron depletion on the transcriptome of Enterococcus faecalis

To identify the genes and pathways utilized by E. faecalis to grow under iron starvation, we used RNA deep sequencing (RNA-seq) to compare the transscriptome of the parent strain OG1RF grown to mid-log in the chemically defined FMC medium with or without the addition of FeSO4 as an iron source. E. faecalis OG!RF cells were grown to mid-log phase in chemically defined FMC medium with (Complete) or without (Minus Fe) supplementation with 80uM FeSO4 as a source of iron. Total RNA was isolated and prepared for RNA-Seq analysis.