Genome-wide expression analysis of EPCAM+ epithelial cells from 3-dimensional lung organoids harboring Hermansky-Pudlak Syndrome-associated mutations

Introduction of Hermansky-Pudlak Syndrome-associated mutations with CRISPR/Cas9 genome editing, allows for disease modeling in 3D cultures of hPSC-derived lung organoids. Wild-type human pluripotent stem cells (hPSCs) or hPSCs harboring Hermansky-Pudlak Syndrome-associated mutations introduced by CRISPR/Cas9 genome-editing were differentiated to Day40 3D lung organoids and EPCAM+ epithelial cells were sorted. mRNA profiles from these cells were generated by deep sequencing, in triplicate, using Illumina HiSeq2500.