Transcriptome Profiling of Escherichia coli B During Sequential Adaptation to T4 Phage and Iron (III) Stress DATASET

The Escherichia coli B populations utilized for RNA-seq were derived from the 35-day experimental evolution delineated in Ezeanowai et al. [5]. RNA extraction was performed in five replicates for each of the five experimental groups (Ancestor (ANC), control (CON), phage-selected (Phage), iron (III)-selected (FE), and phage/iron (III)-selected (PF)), using TRIzol to obtain pure, high-quality RNA. Sequencing was performed on the Illumina NextSeq 1000/2000 platform. Reads were aligned to the E. coli B ATCC 11303 reference genome, and pairwise comparisons, PCA, and heatmap between the five experimental groups were conducted to determine differential gene expression profiles.