MTG8 interacts with LHX6 to specify cortical interneuron subtype identity

In the study we compared migrating embryonic cortical interneurons from control mouse embryos and ones carryng homozygous deletions of either Mtg8 or Lhx6. The aim was to identify genes that are co-regulated by LHX6 and MTG8. RNA was isolated from fluorescent cells that had been sorted from control and mutant embryonic cortices. The cDNA was used to prepare libraries using the Nextera XT kit (Illumina). These were run on a NextSeq 500 sequencer (lllumina). Raw sequence data were adapter and quality trimmed (fastp v0.20.1), aligned to the genome with STAR (v2.7b) and reads per transcript determined with FeatureCounts (v1.6.4).本研究比较了对照小鼠胚胎与携带Mtg8或Lhx6纯合缺失的小鼠胚胎来源的迁移性胚胎皮层中间神经元，旨在鉴定受LHX6与MTG8共同调控的基因。研究人员从对照及突变型胚胎皮层中分选得到的荧光标记细胞中提取RNA，以此合成cDNA并使用Illumina公司的Nextera XT建库试剂盒（Nextera XT kit）制备测序文库。随后采用Illumina NextSeq 500测序仪（NextSeq 500 sequencer）完成测序。原始测序数据通过fastp v0.20.1进行接头切除与质量修剪，利用STAR v2.7b将测序reads比对至参考基因组，并通过FeatureCounts v1.6.4统计每个转录本的reads计数。