Results of immunohistochemical stainings of teratomas grown in various hosts.

Teratomas were obtained after injection of ES cells or differentiated (diff.) cells into syngeneic 129Sv mice, immunodeficient SCID/beige mice or LOU/c rats. Some recipients received CsA (10 mg/kg/day). Histological sections were stained by immunohistochemistry for OCT3/4, Ki67, T cells (anti-mouse or anti-rat CD3), B cells (anti-mouse CD45R/B220 or anti-rat CD45R), and macrophages (anti-mouse Mac3 or anti-rat CD68). The presence of the markers is indicated semi quantitatively in four categories: − negative, + single positive cells, ++ groups of positive cells, +++ confluent groups of positive cells. Five representative teratomas per group of mice and 9 teratomas of LOU/c rats were analyzed.
1One not rapidly growing tumor in each of these groups was negative for the marker.
2not tested.
3Teratomas grown in LOU/c rats were stained for infiltrating rat T and B cells as well as rat macrophages.