Small-Angle X-ray Scattering (SAXS) Data of OmpLA Reconstituted in POPC and DLPC Proteoliposomes

This dataset contains Small-Angle X-ray Scattering (SAXS) profiles for Large Unilamellar Proteoliposomes (PLUVs) containing Outer Membrane Phospholipase A (OmpLA). The liposomes were prepared via detergent removal by dialysis, using lipid systems of either POPC + 5 mol% POPG or DLPC + 5 mol% DLPG. Data Acquisition Two distinct beamline/instrument setups were utilized depending on the lipid system: POPC-based samples: Measured at the BioSAXS beamline P12 (EMBL Hamburg, PETRA III, DESY, Germany). Samples (~7 mg/mL) were flowed through a 1 mm quartz capillary at 37°C. Data reduction was performed on-site using standard EMBL pipelines. Targeted protein/lipid molar ratios included 1/900, 1/600, and 1/300. DLPC-based samples: Measured using a SAXSpoint 5.0 system (Anton Paar, Graz, Austria) equipped with a MetalJet X-ray generator and an Eiger R 1 M detector. Samples (~20 mg/mL) were held in an Anton Paar μCell at 37°C. Data integration and background subtraction were performed using SAXSanalysis software (Anton Paar). Targeted ratios included 1/1200 and 1/300. Data Contents Files within PLUV_SAXS.zip : DLPC_OMPLA_PL_300_sub_merged_nmA.dat DLPC_OMPLA_PL_1200_sub_merged_nmA.dat DLPC_reference_sub_merged_nmA.dat POPC_OmpLA_PL_300_sub_nmA.dat POPC_OmpLA_PL_600_sub_nmA.dat POPC_OmpLA_PL_900_sub_nmA.dat POPC_reference_sub_nmA.dat The uploaded files include: Scattering curves: 3 space-separated columns (*.dat) containing: 1. q-vector (Å⁻¹) 2. Scattering intensity I(q) (arb.u.) 3. Intensity uncertainty / standard deviation Background-subtracted curves: Processed data ready for analysis. Reference systems: Mock control data (lipid-only vesicles processed identically to protein samples). Buffer composition: 20 mM TRIS buffer + 2 mM EDTA. Nomenclature PL: protein to lipid molar ratio (e.g. DLPC_OMPLA_PL_300: OmpLA in DLPC vesicles at a molar ratio of 1/300) POPC: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine POPG: 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol DLPC: 1,2-dilauroyl-sn-glycero-3-phosphocholine DLPG: 1,2-dilauroyl-sn-glycero-3-phosphoglycerol Context Full methodological details regarding PLUV preparation and concentration determination can be found in the associated manuscript supplementary materials. Protocols were adapted from Herrmann et al. (2015) and Piller et al. (2023).