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Detection of the B-LCY2 editing by high-throughput sequencing

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NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA853727
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The B-LCY2 CRISPR/Cas9-targeted region of 58 transgenic citrus lines (36 for Doppio Sanguigno, 2 for Vaccaro, 5 for Tarocco TDV, 2 for Tarocco Lempso, 5 for Bud Blood, and 8 for Carrizo citrange) was massively screened by high-throughput sequencing (HTS). The B-LCY2 region containing both the target sites was amplified by specific primers with overhang Illumina adapters.
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2022-06-28
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