Spanish Human Proteome Project: Dissection of Chromosome 16

The Chromosome 16 Consortium forms part of the Human
Proteome Project that aims to develop an entire map of the proteins
encoded by the human genome following a chromosome-centric strategy
(C-HPP) to make progress in the understanding of human biology in
health and disease (B/D-HPP). A Spanish consortium of 16 laboratories
was organized into five working groups: Protein/Antibody microarrays,
protein expression and Peptide Standard, S/MRM, Protein Sequencing,
Bioinformatics and Clinical healthcare, and Biobanking. The project is conceived on a multicenter configuration, assuming
the standards and integration procedures already available in ProteoRed-ISCIII,
which is encompassed within HUPO initiatives. The products of the
870 protein coding genes in chromosome 16 were analyzed in Jurkat
T lymphocyte cells, MCF-7 epithelial cells, and the CCD18 fibroblast
cell line as it is theoretically expected that most chromosome 16
protein coding genes are expressed in at least one of these. The transcriptome
and proteome of these cell lines was studied using gene expression
microarray and shotgun proteomics approaches, indicating an ample
coverage of chromosome 16. With regard to the B/D section, the main
research areas have been adopted and a biobanking initiative has been
designed to optimize methods for sample collection, management, and
storage under normalized conditions and to define QC standards. The
general strategy of the Chr-16 HPP and the current state of the different
initiatives are discussed.