Spatial Tumor Proteome Profiling by Immunohistochemistry-Based Laser Capture Microdissection and Data-Independent Proteomics
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https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=f8f12711c0714c259ea4349b664401f1
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资源简介：
We designed an immunohistochemistry (IHC)-based workflow for cell type-resolved proteome analysis of tissue samples. Firstly, targeted cell type was marked by IHC using antibody targeting cell-type specific marker to improve accuracy and efficiency of LCM. Secondly, to increase protein recovery from chemically crosslinked IHC tissues, we optimized a decrosslinking procedure to seamlessly combine with the integrated spintip-based sample preparation technology SISPROT. This newly developed approach, termed IHC-SISPROT, has comparable performance as H&E staining-based proteomic analysis.
创建时间：
2020-03-20
