Richard Allen (University of Hawaii) (2011) CIL:9853, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

The radial arms of the Contractile Vacuole Complex or CVC each contain a collecting canal enclosed by a microtubular ribbon split lengthwise into several smaller ribbons, a meshwork of smooth spongiome and, peripheral to this, the bundles of tubules of the decorated spongiome. The microtubules along the collecting canal are immunogold-labeled with anti-b-tubulin as are some single microtubules in the area. TEM taken on 7/8/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 7,900X. Bar = 1µm. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:36689) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

收缩空泡复合体（Contractile Vacuole Complex，简称CVC）的辐射臂内含有由微管带状结构分割成数条较小的带状结构所包围的收集管、由平滑海绵状组织构成的网络，以及围绕其外围的装饰性海绵状组织管束。收集管沿线的微管通过抗β-微管蛋白的免疫金标记进行了标记，该标记也存在于该区域的某些单个微管中。该透射电镜（TEM）图像由R. Allen于1996年7月8日使用Zeiss 10A透射电子显微镜，在80kV的加速电压下拍摄。负像放大倍数为7,900倍。标尺长度为1微米。细胞在固定前用0.25%戊二醛轻微固定，并用2.3M蔗糖渗透，之后在液氮中冷冻，并在-100°C的温度下制成约75纳米厚度的薄片。从这些制备中获得的冷冻切片随后被解冻、清洗，并暴露于由小鼠或兔/山羊制备的单克隆初级抗体以及复合有胶体金的抗小鼠/兔山羊二级抗体。制备的详细信息详见于《细胞生物学方法》2010年第96卷第143-173页。负像被打印到纸上，然后扫描至Photoshop。此数字化图像可用于定性分析。该图像的未经处理的、高分辨率版本（CIL:36689）存档在图书馆中，可用于定量分析。更多信息可访问（http://www5.pbrc.hawaii.edu/allen/）。