Richard Allen (University of Hawaii) (2011) CIL:9848, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

Immunogold b-tubulin labeled ribbons that extend from the cytopharynx and have discoidal vesicles bound to their kinetic side, i.e., their anterior side relative to the cell’s longitudinal axis (sidedness explained in Fok et al., J. Euk. Microbiol. 2008). TEM taken on 7/5/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 12,000X. Bar = 0.5µm. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:1309) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

免疫金标记的β-微管蛋白带状结构，自细胞胞咽部延伸而出，并与其运动面相连接的扁囊结构相结合，即相对于细胞的纵向轴而言，其前侧（有关侧向性的解释见Fok等，J. Euk. Microbiol. 2008）。于1996年7月5日由R. Allen使用80kV运行的蔡司10A透射电子显微镜拍摄。负像放大12,000倍。标尺长度为0.5微米。细胞经0.25%戊二醛轻轻固定，并用2.3M蔗糖渗透，随后在液氮中冷冻并沿约75纳米厚度进行超薄切片。从这些制备的冷冻切片中，随后进行解冻、洗涤，并暴露于由小鼠或兔/山羊制备的单克隆一级抗体以及与胶体金复合的山羊抗小鼠/兔二级抗体。制备的详细信息详见于《细胞生物学方法》2010年第96卷第143-173页。负像被打印到纸上，然后扫描至Photoshop。此数字化图像可用于定性分析。未经处理的、高分辨率的图像（CIL:1309）存档于图书馆，可供定量分析。更多信息请访问（http://www5.pbrc.hawaii.edu/allen/）。