Pseudomonas putida B6-2 Transcriptome or Gene expression

The overnight cultures of P. putida B6-2 and P. putida BGR4 were inoculated into fresh LB medium with an initial OD600 = 0.05. When the OD600 of the cultures reached 0.6, the cultures in the treatment group were treated with 0.6 mM 4-NQO for 4 h, while the cultures in the control group were non-treated for 4 h. After the completion of the treatment, samples for RNA extraction and RNA-seq were then immediately collected from the bacterial cultures. Three biological replicates of each sample were adopted to ensure data reliability.