Annex supplement

To confirm the phylogenetic position of Broussonetia kaempferi Sieb. var. australis Suzuki., a phylogenomic analysis was performed based on 23 other species from the moraceae and one outgroup（celtis cerasifera） were used to construct phylogenetic tree.. A total of 58 protein-coding genes shared by all species were extracted and were aligned using MUSCLE (Edgar 2004). The maximum likelihood (ML) analysis was conducted using RAxML version 8.0 software (Stamatakis 2014) based on the GTR + GAMMA nucleotide substitution model with 1000 bootstrap replicates. The phylogenetic analysis showed that Broussonetia kaempferi Sieb. var. australis Suzuki was clustered into Broussonetia kaempferi and were closely related to Broussonetia kazinoki ，and Broussonetia papyrifera (Figure 1). is consistent with previous (Chen et al.2021). The complete chloroplast genome of Broussonetia kaempferi Sieb. var. australis Suzuki reported here contributes to the research of evolutionary studies and conservation biology whithin Moraceae.