Response_of_myeloid_lineage_cells_to_cytokines. Response_of_myeloid_lineage_cells_to_cytokines

This study is focused to find new genes involved in Insulin signalling in bone marrow derived macrophages upon treatment with IL-4 and LPS. We will use primary macrophages differentiated from bone-marrow derived hematopoetic stem cells; our aim is to analyze the transcriptome upon anti-inflammatory (IL-4) and inflammatory (LPS) treatment. Total RNA was extracted with RNAEasy kit form Qiagen using manufacturer reccomendations and RIN values for all samples were between 9 and 10.