Identification of RNA Markers in Red Blood Cells for Doping Control in Autologous Blood Transfusion. Identification of RNA Markers in Red Blood Cells for Doping Control in Autologous Blood Transfusion

We conducted an animal experiment and used total RNA sequencing (RNA-Seq) to identify novel RNA markers to detect autologous blood transfusion (ABT) doping within red blood cells (RBCs) as a pilot study before human trials. The total RNA-Seq and bioinformatics were performed using rat RBCs that were stored 10- and 20-day periods with citrate–phosphate–dextrose solution with adenine (CPDA) at 4°C In the results, we identified three significant genes whose expression levels were drastically decreased according to the storage period. Our novel insights may allow future studies to develop a test method for ABT doping. Overall design: RNAs of RBCs purified form the whole bloods were analyzed using total RNA-Seq, at each storage period.