To identify differentially expressed gene in the atrium tissues between wildtype control and the Dnajb6 heterozygous knockout mouse

To seek molecular mechanisms underlying the sick sinu syndrome phenotypes observed in Dnajb6 heterozygous knockout mice, we performed whole transcriptome RNA-sequencing experiments using right atrial tissues isolated from Dnajb6 heterozygous knockout mic and WT control mice at 1 year of age. Overall design: Total RNA was extracted from the right atrium (RA) tissues of 1-year-old Dnajb6+/- heterozygous mutant hearts and WT sibling controls. Six total samples (3 biological replicates for each genotype) were submitted for RNA sequencing (Azenta Life Science, NJ). Genes were considered to be differentially expressed between the two groups if they exhibited a greater than 2-fold change and an False Discovery Rate (FDR) of less than 0.05 according to the DESeq approach.