Supplementary Figure 23 Helium ion microscopy (HIM) buffer and non-phosphorylated controls preclude salt precipitation

<b> Helium ion microscopy (HIM) buffer and non-phosphorylated controls preclude salt precipitation. </b>In order to determine that our proteins were forming fractal-like patterns and it was not salt inducing the patterns, a buffer and non-phosphorylated proteins sample controls were used to preclude salt precipitation. (<b>A</b>) Usual HIM square salt crystals on a glass surface. (<b>B</b>) Deposited HNG buffer (50 mM Hepes, 100 mM NaCl, 5% glycerol, pH.7.4, buffer proteins are stored in) on silicon wafer shows no structures on the surface. (<b>C</b>) 3 µM non-pY-AtzAM1 and 2 µM AtzCM1 control shows no fractal-like structures. (<b>D</b>) 3 µM non-pY-AtzAM1 and 1 µM AtzCM1 show no fractal-like structures. All controls demonstrate that fractal structures are formed by phosphorylated protein components.