Decision thresholds for transmission of single and multiple HIV-1 variants

Note: The following decision strategy was applied for each analysis of subject's maximum and mean distances within gag and env: A sample with value exceeding the “Multi” threshold was associated with transmission of multiple HIV-1 variants. A sample with value smaller than the “Single” threshold was associated with transmission of a single HIV-1 variant. A sample with value exceeding the “Single” threshold but less than the “Multi” threshold was considered undetermined in relation to multiplicity of HIV-1 transmission.