Sequences of primers used to TLR7 constructs.
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The N-terminal insert was generated by two PCRs in order to add a HA-tag, using the first PCR product as template for the second PCR. In order to target the C-terminal constructs to the ER, the leader sequence from murine MHC class I was added by synthesis. The leader-coding oligonucleotide was subsequently inserted in front of the C-terminal previously cloned into pcDNA3.1. The full-length mTLR7 construct was mutagenized in its Asn 478 into Gln using the Quick-change mutagenesis kit (Stratagene).
创建时间：
2012-08-16
