Single-cell profiling of liver in offspring with intrauterine hyperglycemia reveals acute-phase response deficiency

Gestational diabetes mellitus (GDM) has long-term effects on the offspring health. Among these chronic diseases, inflammatory profiles play important roles in their development. Assessment for inflammatory status of GDM offspring is limited. Thus, the single cell RNA sequencing (scRNA-seq) was conducted to delineate the inflammatory characteristics of offspring with intrauterine hyperglycemia (IHG). scRNA-seq analysis of mice liver revealed myeloid polarization and multiple lineages developmental retardationwas identified in IHG offspring. Proinflammatory potential of myeloid cells seemed to be deeply imprinted in the liver of adult offspring with IHG. Overall design: To assess the hepatic inflammatory characteristics of offspring with IHG. Livers of fetal male offspring (E18.5) and adult male offspring (8 w) with Ctrl and IHG morther were enrolled for scRNA-seq analysis.