Single cell RNA sequencing of 120h after aggregation mouse gastruloids cultured with different doses of retinol

We quantified the effects of retinol on the cell type composition of gastruloids with different concentrations of retinol (9, 300, 600 and 1200 nM), at 120 hours after aggregation. These gastruloids were generated from the Rarb2.LacZ reporter cell line and cultured according to the standard protocol. For each retinol condition, , 40-45 gastruloids from each experimental group were collected and dissociated into single cells. Live single cells were sorted using FACS (CytoFlex SRT Beckman Coulter). DAPI-negative and singlet populations were gated and collected (Methods). Single-cell RNA sequencing was done with 10X chromium genomics 3' chemistry.