Primers for amplification of Seg-2 from BTV-26 in RT-PCR assays.

*Individual primers are identified by the BTV serotype (e.g. BTV-26) followed by the letter S and number 2 (to indicate Seg-2), then a number to indicate the relative nucleotide position of the primer within VP2 gene, followed by F or R to indicate forward or reverse orientation.†Primer-pairs 1 and 2 also generated very faint but near right sized bands from Seg-2 of certain serotypes within nucleotype ‘A’ (BTV-4, 10, 17, 20 and 24 – Primer-pair 1; BTV-4, 10 and 17 – Primer-pair 2), the most closely related nucleotype/serotypes to BTV-26 and therefore they cannot be regarded as BTV-26 specific.‡Primer-pairs 3 and 4 although generated multiple bands of low intensity with some serotypes in the nucleotype ‘A’ but none of them was of right size, so these two sets can be regarded as BTV-26 specific.