Nanolipoprotein mediated Her2 protein transfection induces malignant transformation in human breast acinar cultures. Nanolipoprotein mediated Her2 protein transfection induces malignant transformation in human breast acinar cultures

We describe transcriptomic changes in HMT-3522-S1 cells cultured in the acinar morphogenesis assay in reponse to either empty-nanolipoprotein stimulus, Her2-nanolipoprotien stimulus or control unstimulated cells. Overall design: HMT-3522-S1 nonmalignant human breast epithelial cells were cultured in laminin rich extracellular matrix hydrogels for 10 days in either control/no stimulus condition, in the presence of 5ug/ml nanolipoprotein particles comprised of His-tagged Apolipoprotein A1 and 1,2-dimyristoyl-sn-glycero-3-phosphocholine, or in the presence of 5ug/ml nanolipoprotein particles containing the above plus human full length ErbB2 protein (Her2).