Genome wide expression analysis of high passage growth arrested BJ fibroblasts maintained or withdrawn from rapamycin maintenance.

BJ fibroblasts were grown in the presence of rapamycin until reaching growth arrest at high passages, at which point cells were maintained in or withdrawn from rapamycin. RNA was then harvested for RNA-seq BJ fibroblasts were grown to growth arrest in the presence of rapamycin maintenance. Cells were then either maintained in rapamycin or withdrawn from rapamycin for two weeks. Experiments, excepting control treatment, were done in duplicates. RNA-seq tag density was calculated over gene bodies, and each withdrawn samples was compared to its paired maintained sample to determine fold change in gene epression.