Supplement 1. Non-transformed data used in statistical analysis of the effects of temperature and resource utilization on bacterial production in two rivers.

File List EEA_data.txt Description The EEA data.txt file is a tab-separated ASCII file. The file contains the non-transformed data used in statistical analyses of the effects of temperature and resource utilization on bacterial production in two rivers. Rates measured in the lab at 20˚C were converted to rates at ambient temperature using the following equations: BP(T) = BP(T0)e-Ea/k(1/T0-1/T) and AppVmax(T) = AppVmax(T0)e-Ea/k(1/T0-1/T), where BP represents bacterial production, AppVmax is the observed maximum extracellular enzyme activity (EEA) rate, T denotes ambient stream temperature (in K), and T0 equals 293 K (or 20 ˚C); Ea is the activation energy (eV) of the process, and k is Boltzmann's constant (8.62 x 10-5 eV K-1). The activation energy (Ea) was assumed to be 0.5 eV for bacterial production and maximum EEA rates. Column definitions: 1 = Study name; more detailed information can be found at Sinsaubaugh et al. 1997. Limnology and Oceanography 42:29–38. 2 = Ambient temperature (˚C) 3 = Inverse absolute temperature (1/kT) where k is Boltzmann's constant (8.62 x 10-5 eV K-1) and T is ambient temperature in K 4 = Bacterial production (BP, nmol h-1 L-1) 5 = Acetyl-esterase (AE) maximum activity rate (AppVmax) (nmol h-1 L-1) 6 = The half saturation constant (the substrate concentration at which the rate of substrate conversion is equal to Vmax/2) (AppKm, nmol) for AE activity 7 = The turnover rate (St, h-1) of AE defined as AppVmax/2AppKm 8 = Endoprotease (EP) maximum activity rate (AppVmax) (nmol h-1 L-1) 9 = The half saturation constant (the substrate concentration at which the rate of substrate conversion is equal to Vmax/2) (AppKm, nmol) for EP activity 10 = The turnover rate (St, h-1) of EP defined as AppVmax/2AppKm 11 = Leucyl-aminopeptidase (LAP) maximum activity rate (AppVmax) (nmol h-1 L-1) 12 = The half saturation constant (the substrate concentration at which the rate of substrate conversion is equal to Vmax/2) (AppKm, nmol) for LAP activity 13 = The turnover rate (St, h-1) of LAP defined as AppVmax/2AppKm 14 = alpha-1,4-glucosidase (AG) maximum activity rate (AppVmax) (nmol h-1 L-1) 15 = The half saturation constant (the substrate concentration at which the rate of substrate conversion is equal to Vmax/2) (AppKm, nmol) for AG activity 16 = The turnover rate (St, h-1) of AG defined as AppVmax/2AppKm 17 = beta-1,4-glucosidase (BG) maximum activity rate (AppVmax) (nmol h-1 L-1) 18 = The half saturation constant (the substrate concentration at which the rate of substrate conversion is equal to Vmax/2) (AppKm, nmol) for BG activity 19 = The turnover rate (St, h-1) of BG defined as AppVmax/2AppKm 20 = Alkaline phosphatase (AP) maximum activity rate (AppVmax) (nmol h-1 L-1) 21 = The half saturation constant (the substrate concentration at which the rate of substrate conversion is equal to Vmax/2) (AppKm, nmol) for AP activity 22 = The turnover rate (St, h-1) of AP defined as AppVmax/2AppKm 23 = Carbohydrate turnover rate (St, h-1); the sum of BG and AG turnover rates 24 = Protein turnover rate (St, h-1); the sum of LAP and EP turnover rates 25 = Ratio of carbohydrate:protein turnover (dimensionless) 26 = Resource pools contributing to bacterial production defined as the sum of AppVmax/2 for all extracellular enzyme activities (EEA) Missing values are represented as “.”. Check sum values: Column 2: check sum = 962, 0 missing values Column 3: check sum = 2197, 0 missing values Column 4: check sum = 194769, 4 missing values Column 5: check sum = 183177, 1 missing value Column 6: check sum = 1359360, 0 missing values Column 7: check sum = 4.615, 0 missing values Column 8: check sum = 246091, 14 missing values Column 9: check sum = 3954300, 14 missing values Column 10: check sum = 1.59, 14 missing values Column 11: check sum = 50756, 0 missing values Column 12: check sum = 2945410, 0 missing values Column 13: check sum = 0.54, 0 missing values Column 14: check sum = 73194, 4 missing values Column 15: check sum = 3.91, 4 missing values Column 16: check sum = 4733, 1 missing value Column 17: check sum = 124638, 1 missing value Column 18: check sum = 5.96, 1 missing value Column 19: check sum = 24782, 1 missing value Column 20: check sum = 291090, 0 missing values Column 21: check sum = 3.04, 0 missing values Column 22: check sum = 9.87, 0 missing values Column 23: check sum = 2.13, 0 missing values Column 24: check sum = 408, 0 missing values Column 25: check sum = 225418, 14 missing values