Knockout of Psen1, Gsk3Beta, and Bmpr1a genes in primary rat astrocytes to convert them into Neurons (Raw Data)

This repository contains the raw multimodal datasets generated from experiments in which Psen1, Gsk3β, and Bmpr1a were knocked out in primary rat astrocytes to induce neuronal conversion. It provides raw imaging, electrophysiology, sequencing, and protein‑expression data captured directly from experimental workflows. Following is the short description of each files: ICC.zip — Immunocytochemistry Dataset Contents: Fluorescence microscopy images and associated metadata. Includes: Raw ICC images (TIFF) Staining panels (neuronal, glial) Purpose: Validates cellular identity, morphology, and marker expression in experimental samples. MEA.zip — Microelectrode Array Electrophysiology Dataset Contents: Raw and processed MEA recordings. Includes: Spike data files Firing rate matrices Raster plots, network activity summaries Analysis scripts or metadata Purpose: Characterizes neuronal activity, network behavior, and functional responses under experimental conditions (crispr). Sanger_Seq_Raw_Analysis.zip — Genetic Validation Dataset Contents: Raw Sanger sequencing chromatograms and processed sequence analyses. Includes: .ab1 chromatogram files FASTA sequences Alignment outputs Mutation/variant confirmation reports Purpose: Confirms genetic constructs, validates sequence integrity, and analyses knockout score data. Western_Blot.zip — Protein Expression Dataset Contents: Western blot images (Raw and Greyscale) Includes: Raw blot images Protein concentration quantification spreadsheets Purpose: Assesses protein concentration to run the gel. Gel images to quantify for measuring expression of different protein levels.