Proteomic evaluation of the hepatocyte secretome identifies Fetuin B as a factor linking steatosis to impaired glucose metabolism

The experiment examined the changes in gene expression in heptocytes isolated from mice with normal (non-fatty) livers or mice with simple steatosis induced by short-term high-fat feeding (6 weeks) Four biological replicates of RNA samples from isolated hepatocytes derived from C57Bl/6 mice fed a high fat diet (HFD) are compared to four biological replicates of RNA samples from isolated hepatocytes of C57Bl/6 mice fed a normal diet (chow)