Peptidomelanin release assays - underlying data

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Peptidomelanin Release Analysis and Figure Generation:

This repository contains scripts used to analyze melanin release experiments and generate publication-quality figures. The workflow processes experimental absorbance measurements, performs statistical analysis, and produces figure panels suitable for inclusion in manuscripts.
This underlying data contains the following files (besides README):
-Melanin_release.csv: file containing all the raw data for melanin release assays.
-Panel_A.R: R scripts for image generation and statistical analysis of Figure 1A in the main article. Generates the time-course plot of melanin release comparing two fungal strains.. This R script draws input from Melanin_release.csv.
-Panel_B.R: R scripts for image generation and statistical analysys for Figure 1B in the main article. Generates a comparison of melanin release across different nutrient conditions. This R script draws input from Melanin_release.csv.
-commands.sh: A bash/shell wrapper script that runs Panel_A.R and Panel_B.R. It will concatenate the image outputs and generate a combined figure (panel_AB.png).
-release.png: Same as panel_AB.png but I manually added annotations (panel labels: A and B). This is the final image that is included in the article.
-panel_A.pdf, panel_B.pdf, panel_A.png, panel_B.png, panel_AB.png are intemediate outputs.
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DEPENDENCIES:

These scripts were run on Ubuntu 22.04.5 LTS (jammy)
bash scripting: Required for installing R, needed for stat. anal. and img. gen.:
$ sudo apt install r-base-core

bash scripting: Required for installing pdf2ppm, for converting pdfs to pngs:
$ sudo apt install poppler-utils

bash scripting: Required for combining images:
$ sudo apt install imagemagick

R scripting: install beeswarm package, needed for jittering datapoints:
>install.packages("beeswarm")