Expression data from 1499 cells, a mouse ADM/PanIN1-derived pancreatic ductal cell line, and the mouse pancreatic ductal adenocarcinoma (PDAC) AH375 cell line.. Mus musculus

In a Kras-driven mouse model of multistage pancreatic cancer progression, decreased p-ERK levels correlate with tumor initiation. In cells derived from this model, transformed cells with low p-ERK levels express markers of pluripotency and demonstrate phenotypes of tumor initiating cells, such as formation of free-floating tumor spheres. Here, a comparison of gene expression from the 1499 cell line, which are premalignant cells isolated from mouse ADM (acinar-to-ductal metaplasia) and PanIN1 (pancreatic intraepithelial lesions), and the AH375 cell line, established from mouse PDAC, was performed. Overall design: To establish the 1499 cell line, cells were isolated from premalignant pancreatic lesions (ADM and PanIN1) in a 9-week-old Pdx1-Cre;LSL-KRasG12D mouse. To establish the AH375 cell line, cells were isolated from PDAC in a 52-week-old Pdx1-Cre;LSL-KRasG12D mouse. These pancreatic ductal cells were propagated on surfaces coated with laminin and in a specific pancreatic medium. Total mRNA extracts were prepared in triplicates for both cell lines and sent to the Génome Québec Innovation Centre for RNA processing and data acquisition.