DNA-seq of yeast tetrads, ChIP-seq and MNase-seq, used for comparing the number of crossover generated per meiosis, DSB intensity and histone occupation between diploid and tetraploid yeast.
We developed a CRISPR-Cas assisted random mutation (CARM) technology for whole genome mutagenesis. As a proof-of-principle, CARM was applied to evolve the capacity of Saccharomyces cerevisiae to produ
we established an efficient HyperTRIBE (Targets of RNA-binding proteins Identified By Editing) in yeast, by fusing a RBP to the hyper active catalytic domain of human RNA editing enzyme ADAR2 and expr
