GnRH pulse generator activity in mouse models of polycystic ovary syndrome

One in ten women in their reproductive age suffer from polycystic ovary syndrome (PCOS) that, alongside subfertility and hyperandrogenism, typically presents with increased luteinizing hormone (LH) pulsatility and secretion. As such, it is suspected that the arcuate kisspeptin (ARNKISS) neurons that represent the GnRH pulse generator are dysfunctional in PCOS. We used here in vivo GCaMP fiber photometry and other approaches to examine the behavior of the GnRH pulse generator in two mouse models of PCOS. We began with the peripubertal androgen (PPA) mouse model of PCOS but found that it had a reduction in the frequency of ARNKISS neuron synchronization events (SEs) that drive LH pulses. Examining the prenatal androgen (PNA) model of PCOS, we observed highly variable patterns of pulse generator activity with no significant differences detected in ARNKISS neuron SEs, pulsatile LH secretion, or serum testosterone, estradiol, and progesterone concentrations. However, an unsupervised machine ..., , , # eLife-97179-GnRH pulse generator activity in mouse models of polycystic ovary syndrome

[https://doi.org/10.5061/dryad.mpg4f4r97](https://doi.org/10.5061/dryad.mpg4f4r97)

## Description of the data and file structure

### Files and variables

#### File: Dryad\_Figure\_Excels-eLife-97179.zip

**Description:** We have submitted 10 Excel files in total corresponding to all 10 figures in the paper in (**Dryad_Figure_Excels-eLife-97179.zip**). Each figure has one Excel sheet with panels corresponding to each tab. For example, *file Fig.1-eLife-97179 tab C* corresponds to *Fig.1C* in the original article.

More details of data collection and anlysis can be found in figure legends and methods section of the original article here [https://doi.org/10.7554/eLife.97179](https://doi.org/10.7554/eLife.97179.2)

**Applicable for all**:

* Species of study: mice
* Missing values are listed as blank;

- Each value represent a mouse (biological replicate);
- PPA: peripubertal androgen- treated anima...