Dual RNA-seq of Neisseria gonorrhoeae and human neutrophils

Deep sequencing of cDNA from Neisseria gonorrhoeae bacteria and human neutrophils, alone and after coincubation. Overall design: Total RNA was isolated from Neisseria gonorrhoeae and adherent primary human neutrophils, alone and after co-culture over time. Bacteria were of strain FA1090 ?opa ("Opaless"; 130), FA1090 ?opa with H041 penA, penB, and mtrR resistance alleles (3x130), or H041. Bacteria were untreated or treated with experimentally determined sublethal concentrations of selected antibiotics 1 hour prior to and during co-culture. Ribosomal RNA was depleted and the remaining RNA was reverse-transcribed into a cDNA library for Illumina sequencing. Reads were mapped to the FA1090, H041 and/or human genomes.