Cellular model of chromosome 3p deletion

Aneuploidy, whole chromosome or chromosome arm imbalance, is a near-universal characteristic of human cancers. Chromosome arm-level alterations show cancer-specific patterns, including loss of chromosome arm 3p in squamous cancers. We applied genome engineering to delete 3p in human lung epithelial cells, causing decreased proliferation rescued in part by chromosome 3 duplication. This study defines provides an experimental approach to study chromosome arm aneuploidy.